• Biophotonics and waves
  • High Tech / Low Cost Technologies
  • Technologies for in vivo biology on animal models

INFLUERE IN VISCERA – Coupling of interferometric microscopy and a fluorescence microscope channel

Project lead by  Claude Boccara,  Marianne De Paepe,  Harry Sokol
Industrial partners  Quattrocento


Recent studies indicate that differences in gut virome composition are markers of Crohn’s disease (CD). In addition, there are more and more reports of the role of membrane vesicles secreted by the gut microbiota and by human cells in this disease. Overall, there are good indications that the type and numbers of viruses and vesicles present in the intestine are indicators of the physiological state of individuals.

Yet a thorough characterization of these differences is still lacking, which is in partly due to important technical difficulties: the very small size and high diversity of viruses and vesicles makes their numeration and description very difficult.

To improve this, we propose to develop an innovative approach that relies on the coupling of interferometric microscopy (IM) and a fluorescence microscope channel. IM can quantify and give the size distribution of all nanoparticles present in complex samples, such as stool, in few minutes, while the fluorescence microscope channel enables the discrimination viruses, prokaryotic vesicles, and eukaryotic vesicles.

In addition, we will use a microfluidic device to enrich/sort in viral particles for further genomic studies.



As a response to the : Call for projects 2018 : Innovative technologies

Development and Dissemination of Innovative Technologies to be Used in Research in Life Sciences

Details & Selected Projects


  • Langevin Institute

    Langevin Institute

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  • Micalis Institute

    Paris Saclay University

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  • Gastroenterology Department

    Hôpital Saint Antoine

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