• Microfluidics
  • Technologies for in vivo biology on animal models

C. elegans development, perturbed in space and time using microfluidics

Project lead by  Wolfgang Keil,  Patrick Tabeling
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Abstract

In this PhD project, we propose to develop and apply a novel live imaging technology to investigate the mechanisms underlying the synchrony of gene expression and stem cell divisions during development of the roundworm Caenorhabditis elegans (C. elegans).

By combining optimized micro-patterned electrical resistor arrays and microfluidics animal confinement, we will develop a system to impose a linear temperature gradient, in which the anterior and posterior halves of the C. elegans larva are developing at different temperatures and thus, at different rates.

Using this technology, through live imaging of gene expression and waves of stem cell divisions as well as mathematical modelling of gene regulatory networks, we will then investigate whether and how compensatory mechanisms can counteract the effects of this unnatural environmental condition.

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Call

As a response to the : Call for projects 2020 : Co-development of innovative technologies & methods

Call for projects 2020 : Co-development of innovative technologies & methods

Details & Selected Projects
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Teams

  • Quantitative Developmental Biology

    CNRS - French National Centre for Scientific Research
    Curie Institute
    Sorbonne University

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  • Microfluidics, MEMS, Nanostructures

    CNRS - French National Centre for Scientific Research
    ESPCI
    Pierre Gilles de Gennes Institute

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