• Biophotonics and waves
  • Technologies for in vivo biology on animal models

HoloCal – High speed volumetric imaging and photostimulation

A

Abstract

Understanding how the brain computes signals from the outside world is one of the major frontiers in neurosciences. It is today possible to record and manipulate the activity of genetically defined neurons in behaving animals to generate and test hypothesis on how neuronal circuits function in physiological or pathological contexts.

2-photon microscopy is a method of choice to take advantage of these molecular tools to image (GCaMPs) or stimulate (opsins) neurons in awake and behaving animals. However, it usually operates on limited, thin, 2D planes which are not reflecting the 3D distribution of the neurons of interest in a brain volume.

Our project is to implement a core facility system enabling 2- photon Bessel beam imaging of calcium transients in neurons while simultaneously stimulating them using 3D holographic 2-photon photostimulation.

This project brings together biology groups across 4 neurosciences institutes and an expert group in neurophotonics, to build a microscope dedicated to the study of cortical circuits.

C

Call

As a response to the : Call for projects 2018 : Equipment support

Support to shared equipment for the development of innovative technologies for Life Sciences

Details & Selected Projects
T

Teams

  • Laboratory of Structural Plasticity

    CNRS - French National Centre for Scientific Research
    Hôpital Universitaire Pitié Salpêtrière
    Inserm
    Institut du Cerveau
    Sorbonne University

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  • Cellular mechanisms of sensory processing

    CNRS - French National Centre for Scientific Research
    Hôpital Universitaire Pitié Salpêtrière
    Inserm
    Institut du Cerveau
    Sorbonne University

    Read more
  • Cellular Physiology of Cortical Microcircuits Team

    CNRS - French National Centre for Scientific Research
    Hôpital Universitaire Pitié Salpêtrière
    Inserm
    Institut du Cerveau
    Sorbonne University

    Read more
  • Wavefront engineering microscopy

    CNRS - French National Centre for Scientific Research
    Inserm
    Institut de la Vision
    Sorbonne University

    Read more
  • Auditory system dynamics and multisensory processing

    Institut de l'Audition
    Paris Saclay University
    Paris-Saclay Institute of Neuroscience (NeuroPSI)

    Read more
  • Neural coding in the auditory system

    Institut de l'Audition

    Read more
  • Genetics and physiology of hearing

    Institut Pasteur

    Read more