As a response to the Call for projects 2017 : Innovative technologies:


STEMCut : Adaptive optics for in vivo photo-ablation and multiphoton imaging

From the manipulation of vertebrate adult neural stem cells in vivo to technology transfer.

Neural stem cells (NSCs) in the vertebrate adult brain are arranged in pools, but the functional relevance of this organization is unknown. Our two teams, specialized respectively in optical development and NSC biology, recently joined forces to put forward the zebrafish as a unique vertebrate permitting to image, through the skin and skull, adult NSCs in vivo in their niche. The present proposal aims to develop, for the first time, a combined methodology of adaptive optics, laser ablation and large-field multiphoton imaging to manipulate these NSCs in situ, and address how population properties impact individual NSC state and fate.