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Call

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Abstract

We propose to develop a microfluidic platform with integrated microelectrode array (MEA) for stimulation and recording of electrophysiological signals of self-organized 3D neuronal networks.

Human induced pluripotent stem cells (iPSCs) will be used to derive brain organoids on the platform (brain-on- chip approach) with controlled microenvironment in order to scrutinize the neural network activities in a simplified model system.

This bottom-up approach will be complementary to the conventional studies with a top-down approach using real brains/brain slices, which are further complex structures. Once the microfluidic/MEA system established, we will analyze the relationships between stimuli and output signals of brain organoids, and study how plasticity can emerge in such a model system.

Interestingly, the same technology will enable broader neurobiology-related investigations, such as drug testing/screening on human brain models.

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Teams

  • Pole Microfluidics – CNRS : National science research center and École Normale Supérieure and Sorbonne University
  • Axonal degeneration and regeneration – CNRS : National science research center and Inserm and Institut de Biologie Paris-Seine (IBPS) and Sorbonne University