Exchange of exosomes constitutes original intercellular communication pathways in major physio- pathological processes. Identifying target cells and functions of exosomes in vivo is of prime importance to strengthen our understanding of exosome communication but also for their future use as biomarkers and as therapeutics.
We have unlocked a scientific and technological latch by developing an in vivo model in zebrafish embryos to track individual endogenous exosomes in the whole organism. To further investigate their fates and functions, we propose to set up a reproducible workflow of in vivo dynamic Correlative Light and Electron Microscopy (CLEM) on this animal model. The realization of this project requires significant microscopy and software developments.
Correlating functional and ultrastructural information will help identifying exosome targets and cell biology processes supporting this fundamental mechanism of communication, thus paving the way for exosome clinical application.
- Endosomal dynamics in neuropathies – Inserm and Institute of Psychiatry and Neuroscience of Paris (IPNP) and University Paris Descartes
- Structure and membrane compartments – CNRS : National science research center and Curie Institute and Sorbonne University
- Space-time Imaging of Organelles and Endomembranes Dynamics – CNRS : National science research center and Sorbonne University