We aim to develop a cell culture platform to automatically differentiate human induced pluripotent stem cells (hiPSCs) for lab-on-a chip applications.
This platform will consist of a culture unit, placed in an incubator for the circulation of the culture medium, a cold unit for storing and supplying the medium and cell factors to the circulating loop, and a control unit for accurate and long term regulation.
In vivo-like culture substrate made of monolayer nanofibers and microfluidic circulatory system will be used for organ tissue generation.
Such a platform will be flexible, versatile and compatible but different from conventional tools, which are mostly for 2D and static culture. Here, the medium exchange is impulse perfusion-diffusion based and can be operated automatically and reproducibly at high resolution.
By using this platform, time and efforts will be reduced and both cardiac and alveolar models will be studied for tissue repairing assays.