• Biophotonics and waves
  • Single Cell / Single Molecule
  • Technologies for in vivo biology on animal models

O P I – Oblique-Plane Imaging for optical sectioning and fast volumetric imaging : studying the cell nucleus in whole with molecular resolution

Project lead by  Ignacio Izzedin,  Antoine Coulon


Elucidating the functional organization and dynamics of the nucleus in live cells is a major challenge in cell biology to understand gene regulation. Single-molecule based approaches have revealed themselves instrumental to this end. However, these techniques are usually hindered by the lack of optical sectioning and a limited axial range, severely limiting the density and volume of data one can acquire on single nuclei at the molecular level.

In this project, we propose to use adaptive optics and point-spread function (PSF) shaping techniques to obtain fast (millisecond-range), full volumetric, in-focus imaging of entire nuclei. With the help of a deformable mirror, we will develop oblique-plane imaging compatible with angled illumination of the sample, effectively obtaining a single-plane illumination microscopy configuration compatible with high NA objectives, and also capable of fast refocusing.

The expertise and compatibility of the partners involved assure the feasibility of this ambitious project.

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Plate-forme Transcriptome et Epigénome

As a response to the : Call for projects 2019 : Equipment support for the development of innovative technologies to support research in Life Sciences

Call for projets 2019 : Equipment support

Details & Selected Projects


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