isoModLoc – Isotropic Modulated localization to decipher multiple proteins 3D organization at the nanoscale

Modulated Localization Microscopy (ModLoc) represents a radically new approach to encode information from individual emitters in super-resolution microscopy. The modulation of the excitation makes it possible to retrieve axial localization with a uniform precision up to several ten of micrometers in depth, in contrary to standard techniques which failed usually after the first micron within the sample. This modulated information is extracted by an original multiplexed lock-in detection taking full advantage of the specificities of super-resolution microscopy without compromising the acquisition time.


The goal of the project is to develop an isotropic configuration to reach a sub 4 nm isoprecision, and combine with a demultiplexing strategy to reveal 3 dyes simultaneously. Multicolor 3D imaging isoresolution will be developed in the context of the nanoscale architecture of microtubules. The proposed developments will also permit to reach an advanced TRL level.